Analysis of benzo[c]phenanthridine alkaloids in Eschscholtzia californica cell culture using HPLC-DAD and HPLC-ESI-MS/MS
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HPLC Analysis and Biochemical Characterization of LOX from Eschscholtzia californica Cham.
BACKGROUND Plant lipoxygenases (LOXs, EC 1.13.11.12) are involved in lipid degradation, regulation of growth and development, senescence, and defence reactions. LOX represents the starting enzyme of the octadecanoid pathway. The aim of the work was to purify LOX from California poppy (Eschscholtzia californica Cham.), to determine its biochemical properties and to identify and quantify the prod...
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An HPLC–DAD–ESI–MSn method has been developed for simultaneous quantification of eight major compounds in eight Saussurea species which have long been used as the traditional Tibetan medicines. The method was validated for sensitivity, precision, and accuracy. LODs were from 0.11 to 5.01 μg mL−1, overall intra-day and inter-day variation was less than 2.70%, and overall recovery was over 98.0%....
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Pilocarpine, an important imidazole alkaloid, is extracted from the leaves of Pilocarpus microphyllus (Rutaceae), known in Brazil as jaborandi and used mainly for the treatment of glaucoma. Jaborandi leaves also contain other imidazole alkaloids, whose pharmacological and physiological properties are unknown, and whose biosynthetic pathways are under investigation. In the present study, a HPLC ...
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Eschscholtzia californica, Suspension Cultures, Benzophenanthridine Alkaloids, Phenolics, Media Effects Suspension cultures of Eschscholtzia californica accumulate the dihydro forms of the benzo phenanthridine alkaloids sanguinarine, chelirubine, macarpine and chelerythrine, all of which are known to be constituents of the Eschscholtzia plant. Under most experimental conditions dihydrochelirub...
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Osmanthus fragrans Lour. has traditionally been a popular ornamental plant in China. In this study, ethanol extracts of the leaves of four cultivar groups of O. fragrans were analyzed by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) and high-performance liquid chromatography with electrospray ionization and mass spectrometry (HPLC-ESI-MS). The results sugg...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2014
ISSN: 0916-8451,1347-6947
DOI: 10.1080/09168451.2014.917264